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Distribution of neuronal nitric oxide <t>synthase</t> positive <t>(nNOS+)</t> immunoreactivity. (A) nNOS+ cells were fewer in the transition zone (TZ) of the colonic pseudo-obstruction (CPO) colons, which appeared to be relatively dense aboral to the TZ region. (B) Comparison of nNOS+ cell counts between different regions indicates that loss of nNOS+ cells was less severe at locations further away from the TZ region. PD, proximal dilation; T, transverse colon; S, non-dilated sigmoid colon. Scale bar 40 μm. * P < 0.05, ** P < 0.01.
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Distribution of neuronal nitric oxide <t>synthase</t> positive <t>(nNOS+)</t> immunoreactivity. (A) nNOS+ cells were fewer in the transition zone (TZ) of the colonic pseudo-obstruction (CPO) colons, which appeared to be relatively dense aboral to the TZ region. (B) Comparison of nNOS+ cell counts between different regions indicates that loss of nNOS+ cells was less severe at locations further away from the TZ region. PD, proximal dilation; T, transverse colon; S, non-dilated sigmoid colon. Scale bar 40 μm. * P < 0.05, ** P < 0.01.
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Becton Dickinson anti-nos [endothelial (enos), neuronal (nnos) inducible (inos) nitric oxide synthases
Distribution of neuronal nitric oxide <t>synthase</t> positive <t>(nNOS+)</t> immunoreactivity. (A) nNOS+ cells were fewer in the transition zone (TZ) of the colonic pseudo-obstruction (CPO) colons, which appeared to be relatively dense aboral to the TZ region. (B) Comparison of nNOS+ cell counts between different regions indicates that loss of nNOS+ cells was less severe at locations further away from the TZ region. PD, proximal dilation; T, transverse colon; S, non-dilated sigmoid colon. Scale bar 40 μm. * P < 0.05, ** P < 0.01.
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Auspep Pty antiserum to neuronal nos (nnos)
Distribution of neuronal nitric oxide <t>synthase</t> positive <t>(nNOS+)</t> immunoreactivity. (A) nNOS+ cells were fewer in the transition zone (TZ) of the colonic pseudo-obstruction (CPO) colons, which appeared to be relatively dense aboral to the TZ region. (B) Comparison of nNOS+ cell counts between different regions indicates that loss of nNOS+ cells was less severe at locations further away from the TZ region. PD, proximal dilation; T, transverse colon; S, non-dilated sigmoid colon. Scale bar 40 μm. * P < 0.05, ** P < 0.01.
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Biomol GmbH rat neuronal nos (nnos)
Distribution of neuronal nitric oxide <t>synthase</t> positive <t>(nNOS+)</t> immunoreactivity. (A) nNOS+ cells were fewer in the transition zone (TZ) of the colonic pseudo-obstruction (CPO) colons, which appeared to be relatively dense aboral to the TZ region. (B) Comparison of nNOS+ cell counts between different regions indicates that loss of nNOS+ cells was less severe at locations further away from the TZ region. PD, proximal dilation; T, transverse colon; S, non-dilated sigmoid colon. Scale bar 40 μm. * P < 0.05, ** P < 0.01.
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Image Search Results


Distribution of neuronal nitric oxide synthase positive (nNOS+) immunoreactivity. (A) nNOS+ cells were fewer in the transition zone (TZ) of the colonic pseudo-obstruction (CPO) colons, which appeared to be relatively dense aboral to the TZ region. (B) Comparison of nNOS+ cell counts between different regions indicates that loss of nNOS+ cells was less severe at locations further away from the TZ region. PD, proximal dilation; T, transverse colon; S, non-dilated sigmoid colon. Scale bar 40 μm. * P < 0.05, ** P < 0.01.

Journal: Journal of Neurogastroenterology and Motility

Article Title: Molecular and Cellular Characteristics of the Colonic Pseudo-obstruction in Patients With Intractable Constipation

doi: 10.5056/jnm15048

Figure Lengend Snippet: Distribution of neuronal nitric oxide synthase positive (nNOS+) immunoreactivity. (A) nNOS+ cells were fewer in the transition zone (TZ) of the colonic pseudo-obstruction (CPO) colons, which appeared to be relatively dense aboral to the TZ region. (B) Comparison of nNOS+ cell counts between different regions indicates that loss of nNOS+ cells was less severe at locations further away from the TZ region. PD, proximal dilation; T, transverse colon; S, non-dilated sigmoid colon. Scale bar 40 μm. * P < 0.05, ** P < 0.01.

Article Snippet: Cryostat sections were cut to 20 μm, which were then pre-incubated in 1% bovine serum albumin for 1 hour to reduce non-specific binding and incubated at 4°C overnight with antibodies against neuronal nitric oxide synthase (nNOS) (1:500 dilution; BD Transduction Laboratories, San Jose, CA, USA), vasoactive intestinal peptide (VIP) (1:400 dilution; Millipore, Billerica, MA, USA), vesicular acetylcholine transporter (vAChT) (1:200 dilution; Phoenix Pharmaceuticals, Burlingame, CA, USA) and substance P (SP) (1:400 dilution; Millipore).

Techniques: